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KMID : 1134820090380040479
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Journal of the Korean Society of Food Science and Nutrition
2009 Volume.38 No. 4 p.479 ~ p.485
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Modification of Palm Mid Fraction with Stearic Acid by Enzymatic Acidolysis Reaction
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Jeon Mi-Sun
Lee Yoon-Jeong
Kang Ji-Hyun
Lee Jeung-Hee
Lee Ki-Teak
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Abstract
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The acidolysis was performed to produce structured lipid with palm mid fraction (PMF) and stearic acid for 7, 24, and 36 hr at 70oC. The reaction was catalyzed by lipozyme TLIM (immobilized lipase from Thermonyces lanuginosa, amount of 10% and 20% by weight of total substrates) in the shaking water bath. The reaction conditions for maximum incorporation of stearic acid on the structured lipid were obtained when molar ratio of PMF and stearic acid was 1:2; concentration of lipozyme TLIM was 20wt%; reaction temperature was 70℃; and reaction time was 36 hr. After reaction under this condition, incorporation of stearic acid in the structured lipid was obtained up to 36.3% while the major components of triacylglycerol were 1,2-dipalmitoyl-3-stearoylglycerol (PPS, 28.19 area%), 1-palmitoyl-2-oleoyl-3-stearoylglycerol (POS/PSO, 20.70 area%) and 1-palmitoyl- 2,3-distearoylglycerol (PSS, 18.13 area%). However, the fatty acid composition at the sn-2 position suggested that the positional specificity of lipozyme TLIM was not observed due to the acyl migration.
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KEYWORD
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palm mid fraction, stearic acid, acidolysis, lipozyme TLIM, acyl migration
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